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cagA/vacA Analysis

This tool can be used to predict the genotype of specific region in 2 virulence genes in H.pylori, EPIYA motif of CagA and (s1/s2) and (m1/m2) motifs of VacA from the genome sequence in FASTA format.

STEP 1 - Upload Sequence
Supported format: FASTA
STEP 2 - Submit your job

AMR Detection

This tool can be used to determine resistance of H.pylori strains to 2 antibiotics, Clarithromycin and Quinolone base on specific acknowledged mutations in 23s rRNA and gyrA genes in H.pylori from the genome sequence in FASTA format.

STEP 1 - Upload Sequence
Supported format: FASTA
STEP 2 - Submit your job

Genome Comparative Analysis

STEP 1 - Choose strains

You may control-click (Windows) or command-click (Mac) to select more than one.

STEP 2 - Enter job id (optional)

If you want to use your genomes, please annotate it first using RAST and enter job id here.


STEP 3 - Submit

RAST

STEP 1 - Upload Sequence
Supported format: FASTA
STEP 2 - Submit your job

cagA and vacA protein sequence alignment


Basic Local Alignment Search Tool

BLAST finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.

STEP 1 - Select BLAST Type
STEP 2 - Enter Query Sequence
Supported format: FASTA
STEP 3 - Enter Subject Sequence
Supported format: FASTA
STEP 4 - Submit your job

Clustal Omega

Clustal Omega is a new multiple sequence alignment program that uses seeded guide trees and HMM profile-profile techniques to generate alignments between three or more sequences.

STEP 1 - Enter your input sequences
Supported format: FASTA
STEP 2 - Set your parameters
STEP 3 - Submit your job

Roary

Roary is a high speed stand alone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by Prokka (Seemann, 2014)) and calculates the pan genome.

STEP 1 - Upload GenBank
Output files from RAST are supported
STEP 2 - Set your parameters
STEP 3 - Submit your job

Snippy

Snippy finds SNPs between a haploid reference genome and your NGS sequence reads. It will find both substitutions (snps) and insertions/deletions (indels).

STEP 1 - Upload Sequence
Supported format: FASTA
STEP 2 - Upload Reference Sequence
Supported format: FASTA, GenBank
STEP 3 - Submit your job

Gene Extractor

Gene Extractor can be used extract gene from input genome based on sample gene sequence.

STEP 1 - Upload Sequence
Supported format: FASTA
STEP 2 - Enter Query Sequence
Supported format: FASTA
STEP 3 - Submit your job

JBrowse

JBrowse is a fast and full-featured genome browser.

STEP 1 - Upload Sequence
Supported format: FASTA, GENBANK
STEP 2 - Submit your job

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